Comparison of the root, leaf and internode transcriptomes in sugarcane (Saccharum spp. hybrids)

• The organs of sugarcane use carbon in different ways, leading to differing ratios insoluble and soluble compounds Transcription aligned closely with differences the biomass makeup components. Large transcriptional were observed among organs, smaller we according maturity. This study may support efforts develop composition better suited various end uses. major source sink (roots, leaves internodes) ( Saccharum spp. hybrids) have roles consequently apportioning into components these organs. performed RNA-seq on two leaves, three internodes (of maturity) roots similar commercial genotypes determine patterns gene expression associated partitioning. A total 18 libraries (in triplicate) generated per genotype, thus yielding ∼0.6 billion high quality reads, an average length 125 bp, covering 90% long-read SUGIT transcriptome. RNA-seq, differential analysis, annotation pathway analysis performed. Transcript active (1st 5th visible dewlap leaves) displayed higher photosynthetic transcripts some protein respiratory transcripts, low most cell-wall synthesis. Additionally, transcription sucrose synthetic (sucrose phosphate synthase phosphatase) was significantly than that all other Storage sinks (middle bottom lower UDP-glucose synthesis from conversion did meristematic (immature root). Both transcript related photosynthesis, far protein, respiratory-related particularly relation Interestingly, for lignin hemicellulose but not immature internodes. Similar profiles across maturity, i.e., between mature internodes, suggested changes select sets genes influence direction partitioning during maturation. finding contrasts large organ types. knowledge